Cell and Molecular Biology Laboratory

Experiment List

  1. Gram’s Staining
  2. Leishman Staining
  3. Giemsa Staining
  4. Identification of given plant, animal and bacterial cells & their components by microscopy
  5. Thin Layer Chromatography
  6. Tryphan Blue Assay
  7. Isolation of microbial DNA
  8. Isolation of genomic DNA
  9. Electrophoresis _ Agarose and Polyacrylamide Gel
  10. Restriction enzyme digestion& Ligation

Equipment List

SL NO INSTRUMENTS NAME QUANTITY
1 UV VISIBLE SPECTROPHOTOMETER 1
2 COOLING CENTRIFUGE 1
3 DIGITAL WATERBATH 1
4 AUTOCLAVE 1
5 LAMINAR AIRFLOW 1
6 HOT AIR OVEN 1
7 WEIGHING BALANCE 1
8 PH METER 2
9 SDS PAGE UNIT 1
10 MICROPIPETTE-20 2
11 UV TRANSILLUMINATER 1

Enzyme Technology Laboratory

Experiment list

  1. Identification of enzymes (amylase, invertase and catalase)
  2. Isolation of Enzyme from different microorganism.
  3. Isolation of alpha amylase from plant source.
  4. To determine Km and Vmax of α Amylase
  5. Determination of specific enzyme activity of α Amylase.
  6. Effect of pH on Enzyme kinetics.
  7. Effect of temperature on Enzyme kinetics.
  8. Identification of Enzyme by different assay.
  9. Purification of enzymes.
  10. Immobilization of Enzyme by sodium Alginate method

Genetic Engineering Laboratory

Experiment list

  1. Isolation of DNA from bacterial cells
  2. Quantitative analysis of DNA using spectrophotometer
  3. Restriction digestion of DNA
  4. Preparation of competent cells
  5. Transformation in E. coli. with recombinant vector
  6. Demonstration of PCR
  7. Southern blotting
  8. Northern blotting

Equipment list

SL NO INSTRUMENTS NAME QUANTITY
1 PCR 1
2 PH METER 2
3 AGAROSE GEL ELECTROPHORESIS UNIT 1
4 DEEP FREEZER (-20 C ) 1
5 REFRIGERATOR 1
6 MICROPIPETTE (100) 2
7 MICROPIPETTE (200) 2
8 MICROSCOPE 3
9 WEIGHING BALANCE 1
10 MINI GEL 1

Developmental Biology Laboratory

Experiment List

  1. Identification of developmental stages of chick embryo using permanent mounts
  2. Preparation of a temporary stained mount of chick embryo
  3. Study of developmental stages of Anopheles.

Plant Biotechnology Laboratory

Experiment list

  1. Preparation of plant tissue culture medium.
  2. Organ culture, Callus

Equipments list

SL NO INSTRUMENTS NAME QUANTITY
1 BIOSAFETY CABINET 1
2 GEL ROCKER 1
3 MICROTOME 1
4 MICROSCOPE 3
5 PH METER 1
6 HOT AIR OVEN 1
7 AUTOCLAVE 1
8 MICROPIPETTE (200) 3
9 REFRIGERATOR 1

Animal Biotechnology Laboratory

Experiment list

  1. Preparation of Ca++ -Mg++ -free phosphate buffered saline at different pH, Preparation of other buffers (HBSS, MES)
  2. Sub-culturing of adherent and non adherent cell lines, with counting & viability staining of cells, Cryopreservation and thawing of cells
  3. Primary culture of animal cells: Aseptic techniques, selection and isolation of organ, disaggregation (mechanical/enzymatic), seeding, cell counting and cell viability
  4. Separation of primary cells from blood by Histopaque 1077 and 1119 and Percoll density gradient centrifugation,
  5. Study of apoptosis by microscopy/western blotting and/or FACS, Cytotoxicity assays (MTT and MTS and PMS and real time PCR)

Experiments list

SL NO INSTRUMENTS NAME QUANTITY
1 CO2 INCUBATOR 1
2 INVERTED MICROSCOPE 1
3 AUTOCLAVE VERICAL 1
4 HOT PLATE STIRRER 1
5 PH METER 2
6 MICROPIPETTE (1000) 2
7 WESTERN BLOT UNIT 1
8 DEEP FREEZER (-40) 1
9 MICROWAVE OVEN 1

Immunology Laboratory

Experiment List

  1. Cells and Organs of Immune System: Examine the cells that comprise the immune system (counts and morphology), Locate organs and tissues of immune system (primary and secondary)
  2. Preparation of blood film and identification of cells, Collection of serum, purification of IgG
  3. Polarization of immune cells (phagocytes only) using stimulation cocktail and characterization
  4. Estimation of reactive oxygen species (ROS) by fluorescence dye DCFDA/DHR and reactive nitrogen species (RNS) by griess reaction
  5. Determination of cytokines by ELISA
  6. Hemagglutination test (HA), Hemagglutination Inhibition test (HI), ELISA etc